Since 2003, PharmOptima has been advancing drug discovery by providing in vitro and in vivo contract research services. PharmOptima, utilizing over 12,000 square feet of laboratory space including a 7,000 square foot vivarium, is a leading CRO conducting ocular research in addition to being recognized as a premier provider of Spinal Muscular Atrophy research. The company’s in vivo research services include delivery, efficacy, tolerance, pharmacokinetics and ADME. PharmOptima’s in vitro expertise includes biochemistry, protein cloning and expression, cell-based assays, immunosorbent assays (traditional ELISA’s and electrochemiluminescent MSD formats) and biomarker research. 
PharmOptima's Core Expertise Drug Development Services - full range of pharmacokinetic and ADME services for IND/NDA
- vivarium with skilled technical staff
- LC-MS/MS method development and validation
- FDA Crystal City guidelines
- GLP compliant sample analysis
Ophthalmic Research Services - pharmacokinetics/ADME
- pharmacology and biochemistry
- specialized dosing
- specialized tissue and fluid collection
- precise sample processing and bioanalysis
- biomarker assays
- MSD electrochemiluminescent
- standard ELISA
Ocular Efficacy Models - VEG-F induced retinal vascular leakage (wet AMD)
- blue light damage (dry AMD)
- STZ induced diabetic retinopathy
- laser induced CNV
- LPS induced Uveitis
- allergic respose
Biochemistry Services - cell-based assays
- protein expression
- prokaryotic (E.coli)
- eukaryotic (mammalian, insect, baculovirus)
- protein purification
- ligand binding assays
- isothermal denaturation
- enzyme inhibitor characterization and mechanism
- fluorescence polarization and other fluorescence based assays
- biomarker assays
- ELISA
- MSD electrochemiluminescent
Bioanalytical Services - rapid screen methods
- method development and validation
- preclinical pharmacokinetics
- PK/PD assessment
- toxicokinetic investigations of absorption and clearance
- bioavailability/bioequivalence
- ocular PK
- cassette (n-in-1) dosing and analysis
- prodrug and metabolite PK
Assays and Assay Development - ELISA
- traditional (HRP, AP)
- ECL (Meso Scale Discovery format) technologies
- development of multiplex ELISAs using MSD technology
- cell-based assays: signal transduction (eg cAMP)
- ligand binding assays
- radio-isotope
- isothermal denaturation
- fluorescence intensity and polarization
- enzyme assays and kinetics
- inhibitor mechanism and validation
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